NASA Spaceline Current Awareness List #616 19 September 2014

Status Report From: Spaceline
Posted: Friday, September 19, 2014

Papers deriving from NASA support:


Latchney SE, Rivera PD, Mao XW, Ferguson VL, Bateman TA, Stodieck LS, Nelson GA, Eisch AJ.

The effect of spaceflight on mouse olfactory bulb volume, neurogenesis, and cell death indicates the protective effect of novel environment.

J Appl Physiol (1985). 2014 Jun 15;116(12):1593-604. Epub 2014 Apr 17.

(PIs: A.J. Eisch, G.A. Nelson/NSCOR)

Note:STS-135 results.

Journal Impact Factor:3.434

Funding:"BioServe Space Technologies and Kennedy Space Center support staff helped organize and oversee this project (funding support from Amgen, BioServe, and NASA-NNJ10GA25A). The tissue experiments presented here are supported by grants to A. J. Eisch from the National Institutes of Health (DA-016765) and NASA (NNX12AB55G) and NASA NNX10AD59G to G. A. Nelson. S. E. Latchney and P. D. Rivera are supported by Diversity Fellowships (DA 016765-09S1)."


Swift JM, Swift SN, Allen MR, Bloomfield SA.

Beta-1 adrenergic agonist treatment mitigates negative changes in cancellous bone microarchitecture and inhibits osteocyte apoptosis during disuse.

PLoS One. 2014 Sep 11;9(9):e106904.

(PI: S.A. Bloomfield)

Note:Hindlimb unloading study. This article may be obtained online without charge.

Journal Impact Factor:3.534

Funding:"These studies were funded through the NASA Cooperative Agreement NCC 9-58 with the National Space Biomedical Research Institute (SAB), the Sydney and JL Huffines Institute for Sports Medicine and Human Performance (JMS), and the Texas Chapter of the American College of Sports Medicine (JMS). JMS was supported by a National Space Biomedical Research Institute Graduate Fellowship NSBRI-RFP-05-02."


Borak TB, Chapman PL.

Description and verification of an algorithm for obtaining microdosimetric quantities for high-LET radiation using a single TEPC without pulse height analysis.

Radiat Res. 2014 Sep 11. [Epub ahead of print]

(PI: T.B. Borak)

Journal Impact Factor:2.445

Funding:"Support for this project was provided by the National Space Biomedical Research Institute (NSBRI) through grant RE01301 at Colorado State University, and research grant NNX13AD19G for Early Stage Innovations (ESI) administered through the NASA Space Technology Research Grants Program."


Sweet TB, Panda N, Hein AM, Das SL, Hurley SD, Olschowka JA, Williams JP, O'Banion MK. Central nervous system effects of whole-body proton irradiation.

Radiat Res. 2014 Jul;182(1):18-34. Epub 2014 Jun 17.

(PI: M.K. O'Banion)

Journal Impact Factor:2.445

Funding:"Support for this work was provided by grants NXX08BA09G and NXX13AC33G from the National Aeronautics and Space Administration."


Wang H, Wang X, Chen G, Zhang X, Tang X, Park D, Cucinotta FA, Yu DS, Deng X, Dynan WS, Doetsch PW, Wang Y.

Distinct roles of Ape1 protein, an enzyme involved in DNA repair, in high or low linear energy transfer ionizing radiation-induced cell killing.

J Biol Chem. 2014 Sep 10. pii: jbc.M114.604959. [Epub ahead of print]

(PI: Y. Wang/NSCOR)

Note:This article may be obtained online without charge.

Journal Impact Factor:4.6

Funding:"This work was supported by grants from the National Aeronautics and Space Administration (NNX11AC39G) and the National Institutes of Health (P30CA138292)." [Editor's note: grant number is NNX11AC30G.]


Latchney SE, Masiulis I, Zaccaria KJ, Lagace DC, Powell CM, McCasland JS, Eisch AJ.

Developmental and adult GAP-43 deficiency in mice dynamically alters hippocampal neurogenesis and mossy fiber volume.

Dev Neurosci. 2014;36(1):44-63.

(PI: A.J. Eisch)

Note:This article may be obtained online without charge.

Journal Impact Factor:2.453

Funding:"This work was supported by grants to A.J.E. from the NIH (DA 0167565, DA 016765-07S1, DA 023555) and NASA (NNX-12AB55G). S.E.L. is supported by a Diversity Fellowship (DA 016765-09S1) from AJE's NIH R01 grant. I.M. was supported by a postdoctoral fellowship on a Ruth L. Kirschstein National Research Service Award (NRSA) Institutional Research T32 Training Grant (T32DA 007290) from the NIDA."


Walker AK, Rivera PD, Wang Q, Chuang JC, Tran S, Osborne-Lawrence S, Estill SJ, Starwalt R, Huntington P, Morlock L, Naidoo J, Williams NS, Ready JM, Eisch AJ, Pieper AA, Zigman JM.

The P7C3 class of neuroprotective compounds exerts antidepressant efficacy in mice by increasing hippocampal neurogenesis.

Mol Psychiatry. 2014 Apr 22. [Epub ahead of print]

(PI: A.J. Eisch)

Journal Impact Factor:15.147

Funding:"These studies were made possible through funding from the NIH (1R01MH085298 and 1R01DA024680 to JMZ; T32DA007290 to AKW through AJE, DA016765 and DA023555 to AJE and 1RO1MH087986 to AAP and Steven L McKnight), institutional funds from University of Iowa Carver College of Medicine to AAP, an International Research Alliance with the Novo Nordisk Foundation Center for Basic Metabolic Research at the University of Copenhagen (to JMZ), the Edward N and Della C Thome Memorial Foundation (to JMR), the Welch Foundation (I-1612; to JMR), NASA (NNX12AB55G to AJE) and an unrestricted endowment provided to Steven L McKnight by an anonymous donor."


Qin YX, Hu M.

Mechanotransduction in musculoskeletal tissue regeneration: Effects of fluid flow, loading, and cellular-molecular pathways.

Biomed Res Int. 2014;2014:863421. Epub 2014 Aug 18. Review.

(PI: Y.X. Qin)

Note:This article may be obtained online without charge.

Journal Impact Factor:2.706

Funding:"This work is kindly supported by the National Institute of Health (R01 AR52379 and R01AR61821), the US Army Medical Research and Materiel Command, the National Space Biomedical Research Institute through NASA Contract NCC9-58."


Burgado J, Harrell CS, Eacret D, Reddy R, Barnum CJ, Tansey M, Miller A, Wang H, Neigh GN.

Two weeks of predatory stress induces anxiety-like behavior with co-morbid depressive-like behavior in adult male mice.

Behav Brain Res. 2014 Sep 6. pii: S0166-4328(14)00579-8. [Epub ahead of print]

(PI: H. Wang)

Journal Impact Factor:3.391

Funding:"Funding for the studies in this manuscript was provided by the NASA grant NNX08BA08G. Salary support for J Burgado was provided by the NIH grant R25GM097636 and salary support for CJ Barnum was provided by the grant for Graduate and Postdoctoral Training in Toxicology 5T32 ES 012870-09."


Other papers of interest:


Katsuda S, Yamasaki M, Waki H, Miyake M, O-Ishi H, Katahira K, Nagayama T, Miyamoto Y, Hasegawa M, Wago H, Okouchi T, Shimizu T.

Spaceflight affects postnatal development of the aortic wall in rats.

Biomed Res Int. 2014;2014:490428. Epub 2014 Aug 19.

Note:STS-90 results. This article may be obtained online without charge.


Mulder E, Linnarsson D, Paloski WH, Rittweger J, Wuyts FL, Zange J, Clment G.

Effects of five days of bed rest with and without exercise countermeasure on postural stability and gait.

J Musculoskelet Neuronal Interact. 2014 Sep;14(3):359-66.

Note:Bed rest study organized by European Space Agency.


Biggar Y, Storey KB.

Global DNA modifications suppress transcription in brown adipose tissue during hibernation.


Rubenstein DA, Yin W.

Hypergravity and hypobaric hypoxic conditions promote endothelial cell and platelet activation.

High Alt Med Biol. 2014 Sep 11. [Epub ahead of print]

Note:Centrifugation was used.


Skow RJ, Tymko MM, MacKay CM, Steinback CD, Day TA.

The effects of head-up and head-down tilt on central respiratory chemoreflex loop gain tested by hyperoxic rebreathing.

Prog Brain Res. 2014;212:149-72.

Note:A tilt table was used.


Pfitzer G.

Biomechanical adaptation to hindlimb suspension: It involves not only transcriptional mechansims but also postranscriptional modification of the molecular motor, myosin.

Acta Physiol (Oxf). 2014 Sep 9. [Epub ahead of print] Editorial.

Note:The article comments onActin sliding velocity on pure myosin isoforms from hindlimb unloaded miceby Maffei M, Longa E, Qaisar R, Agoni V, Desaphy JF, Conte Camerino D, Bottinelli R, Canepari M. Acta Physiol (Oxf). 2014 May 29. [see], which appeared in SPACELINE Current Awareness List #602 on June 13, 2014.


Wisdom KM, Delp SL, Kuhl E.

Use it or lose it: Multiscale skeletal muscle adaptation to mechanical stimuli.

Biomech Model Mechanobiol. 2014 Sep 9. [Epub ahead of print] Review.


Morales MG, Abrigo J, Meneses C, Cisternas F, Simon F, Cabello-Verrugio C.

Expression of the Mas receptor is upregulated in skeletal muscle wasting.

Histochem Cell Biol. 2014 Sep 11. [Epub ahead of print]

Note:Hindlimb cast immobilization was used.


Yarrow JF, Ye F, Balaez A, Mantione JM, Otzel DM, Chen C, Beggs LA, Baligand C, Keener JE, Lim W, Vohra RS, Batra A, Borst SE, Bose PK, Thompson FJ, Vandenborne K.

Bone loss in a new rodent model combining spinal cord injury and cast immobilization.

J Musculoskelet Neuronal Interact. 2014 Sep;14(3):255-66.

Note:Hindlimb cast immobilization was used. This article may be obtained online without charge.


Zuo B, Zhu JF, Li J, Wang CD, Zhao XY, Cai GQ, Li Z, Peng J, Wang P, Shen C, Huang Y, Xu J, Zhang XL, Chen XD.

microRNA-103a functions as a mechno-sensitive microRNA to inhibit bone formation through targeting Runx2.

J Bone Miner Res. 2014 Sep 6. [Epub ahead of print]


Zhao F, Li D, Arfat Y, Chen Z, Liu Z, Lin Y, Ding C, Sun Y, Hu L, Shang P, Qian A.

Reloading partly recovers bone mineral density and mechanical properties in hind limb unloaded rats.

Acta Astronaut. 2014 Sep 1. [In Press]

Note:Hindlimb unloading study.


Pyo JH, Park JS, Na HJ, Jeon HJ, Lee SH, Kim JG, Park SY, Jin YW, Kim YS, Yoo MA.

Functional modification ofDrosophilaintestinal stem cells by ionizing radiation.

Radiat Res. 2014 Apr;181(4):376-86. Epub 2014 Apr 4.


Tang Y, Gao F, Guo S, Li F.

Effects of hypobaria and hypoxia on seed germination of six plant species.

Life Sci Space Res. 2014 Aug 22. [In Press]


Yunze S, Shuangsheng G.

Effects of photoperiod on wheat growth, development and yield in CELSS.

Acta Astronaut. 2014 Aug 26. [In Press]

The journalBioMed Research Internationalis putting together a Special Issue entitled "How Microgravity Affects the Biology of Living Systems." The issue, though incomplete, currently contains 12 articles and several preprints. The articles are available free of charge at the publisher's website: The final version will be published in November. Special Issue editors are Mariano Bizzarri, Jack J.W.A. VanLoon, and Monica Monici.

From the Call for Papers: "topics include, but are not limited to:

  • Direct and indirect mechanisms of gravity sensing by cells and tissues
  • Shape, Cytoskeletal, and nucleoskeletal changes in microgravity
  • Changes in molecular pathways upon microgravity exposure
  • How microgravity affects gene expression and gene-regulatory circuits
  • Technological devices and flight opportunities in microgravity-based investigations
  • Experimental models in microgravity: from 2D to 3D cell cultures"

Links to individual articles are provided as are URLs for articles cited in PubMed. Several papers have been cited in previous SPACELINE Current Awareness Lists. They are listed at the end for completeness with the original List number and date.


Gasperi V, Rapino C, Battista N, Bari M, Mastrangelo N, Angeletti S, Dainese E, Maccarrone M.

A functional interplay between 5-lipoxygenase and -calpain affects survival and cytokine profile of human Jurkat T lymphocyte exposed to simulated microgravity.

Biomed Res Int. 2014;2014:782390. Epub 2014 Sep 16.

Note:A rotary cell culture system was used.


Albi E,Curcio F, Lazzarini A, Floridi A, Cataldi S, Lazzarini R, Loreti E, Ferri I, Ambesi-Impiombato FS.

How microgravity changes Galectin-3 in thyroid follicles.

Biomed Res Int. 2014;2014:652863. Epub 2014 Sep 11.

Note:ISS results. The experiment traveled to ISS on STS-128 and returned on STS-129.


Zhang Y, Lau P, Pansky A, Kassack M, Hemmersbach R, Tobiasch E.

The influence of simulated microgravity on purinergic signaling is different between individual culture and endothelial and smooth muscle cell coculture.

Biomed Res Int. 2014;2014:413708. Epub 2014 Aug 28.

Note:A fast rotating clinostat was used.


Sylos-Labini F, Lacquaniti F, Ivanenko YP.

Human locomotion under reduced gravity conditions: Biomechanical and neurophysiological considerations.

Biomed Res Int. 2014;2014:547242. Epub 2014 Aug 28.

Note:Reduced gravity methods discussed include vertical and tilted body weight support systems, subject load devices, supine suspension systems, and a passive gravity balancing system.


Cazzaniga A, Castiglioni S, Maier JAM.

Conditioned media from microvascular endothelial cells cultured in simulated microgravity inhibit osteoblast activity.

Biomed Res Int. 2014;2014:857934. Epub 2014 Aug 19.

Note:A rotating wall vessel was used.


Masiello MG, Cucina A, Proietti S, Palombo A, Coluccia P, D'Anselmi F, Dinicola S, Pasqualato A, Morini V, Bizzarri M.

Phenotypic switch induced by simulated microgravity on MDA-MB-231 breast cancer cells.

Biomed Res Int. 2014;2014:652434. Epub 2014 Aug 18.

Note:A random positioning machine was used.


Mugnai S, Pandolfi C, Masi E, Azzarello E, Monetti E, Comparini D, Voig B, Volkmann D, Mancuso S.

Oxidative stress and NO signalling in the root apex as an early response to changes in gravity conditions.

Biomed Res Int. 2014;2014:834134. Epub 2014 Aug 17.

Note:Rotation, parabolic flight, and a drop tower were used.


Morabito C, Steimberg N, Mazzoleni G, Guarnieri S, Fan-Illic G, Mariggi MA.

RCCS bioreactor-based modelled microgravity induces significant changes on in vitro 3D neuro-glial cell cultures.

Biomed Res Int. 2014 Sep 9. [In Press]

Note:A Rotary Cell Culture System was used.


Fengler S, Spirer I, Neef M, Ecke M, Nieselt K, Hampp R.

A whole-genome microarray study ofArabidopsis thalianasemi-solid callus cultures exposed to microgravity and non-microgravity related spaceflight conditions for 5 days on board of Shenzhou 8.

Biomed Res Int. 2014 Sep 9. [In Press]

Note:Shenzhou 8 results.


Louis F, Deroanne C, Nusgens B, Vico L, Guignandon A.

RhoGTPases as key players in mammalian cell adaptation to microgravity.

Biomed Res Int. 2014 Sep 9. [In Press] Review.

Note:The review examines studies done in real and simulated microgravity.


Thiel CS, Hauschild A, Tauber A, Paulsen K, Raig C, Raem A, Biskup J, Gutewort A, Hrlimann E, Unverdorben F, Buttron I, Lauber B, Philpot C, Lier H, Engelmann F, Layer LE, Ullrich O.

Identification of reference genes in human myelomonocytic cells for gene expression studies in altered gravity.

Biomed Res Int. 2014 Sep 4. [In Press]

Note:Parabolic flight and sounding rocket results.

The following citations appeared in previous Lists:

Ferranti F, Caruso M, Cammarota M, Masiello MG, Scheri KC, Fabrizi C, Fumagalli L, Schiraldi C, Cucina A, Catizone A, Ricci G.

Cytoskeleton modifications and autophagy induction in TCam-2 seminoma cells exposed to simulated microgravity.

Biomed Res Int. 2014;2014:904396. Epub 2014 Jul 17.

Note:A random positioning machine was used. This article was in CA 613, 29 Aug 2014.

Girardi C, De Pitt C, Casara S, Calura E, Romualdi C, Celotti L, Mognato M.

Integration analysis of microRNA and mRNA expression profiles in human peripheral blood lymphocytes cultured in modeled microgravity.

Biomed Res Int. 2014;2014:296747. Epub 2014 Jun 23.

Note:A rotating wall vessel bioreactor was used. This article was in CA 612, 25 Aug 2014.

Lacquaniti F, Bosco G, Gravano S, Indovina I, La Scaleia B, Maffei V, Zago M.

Multisensory integration and internal models for sensing gravity effects in primates.

Biomed Res Int. 2014;2014:615854. Epub 2014 Jul 1. Review.

Note:This article was in CA 609, 1 Aug 2014.

Pandolfi C, Masi E, Voigt B, Mugnai S, Volkmann D, Mancuso S.

Gravity affects the closure of the traps inDionaea muscipula.

Biomed Res Int. 2014;2014:964203. Epub 2014 Jul 15.

Note:Parabolic flight results. From the abstract: "The possible role of the alterations of membrane permeability induced by microgravity on trap movement is discussed." This article was in CA 613, 29 Aug 2014.

Ulbrich C, Wehland M, Peitsch J, Aleshcheva G, Wise, P, van Loon J, Magnusson N, Infanger M, Grosse J, Eilles C, Sunaresan A, Grimm D.

The impact of simulated and real microgravity on bone cells and mesenchymal stem cells. Biomed Res Int. 2014;2014:928507. Epub 2014 Jul 10. Review.

Note:Reviews studies that used the random positioning machine, 2D-clinostat, and rotating wall vessel bioreactor. This article was in CA 612, 25 Aug 2014.

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